PUBLICATION
10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
- Authors
- Zang, L., Kagotani, K., Nakayama, H., Bhagat, J., Fujimoto, Y., Hayashi, A., Sono, R., Katsuzaki, H., Nishimura, N., Shimada, Y.
- ID
- ZDB-PUB-210323-8
- Date
- 2021
- Source
- Frontiers in cell and developmental biology 9: 588093 (Journal)
- Registered Authors
- Keywords
- Danio rerio, bone regeneration, bone resorption, natural product, osteopenia
- MeSH Terms
- none
- PubMed
- 33748100 Full text @ Front Cell Dev Biol
Citation
Zang, L., Kagotani, K., Nakayama, H., Bhagat, J., Fujimoto, Y., Hayashi, A., Sono, R., Katsuzaki, H., Nishimura, N., Shimada, Y. (2021) 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales. Frontiers in cell and developmental biology. 9:588093.
Abstract
Osteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. However, the anti-osteoclastic components in GHE have not yet been identified. In this study, we separated GHE into several fractions using silica gel column chromatography and evaluated their effects on osteoclastogenesis using a RAW264.7 cell osteoclast differentiation assay (in vitro) and the zebrafish scale model of osteoporosis (in vivo). We identified that the fractions containing 10-gingerol suppressed osteoclastogenesis in RAW264.7 cells detected by tartrate-resistant acid phosphatase (TRAP) staining. In zebrafish, GHE and 10-gingerol suppressed osteoclastogenesis in prednisolone-induced osteoporosis regenerated scales to promote normal regeneration. Gene expression analysis revealed that 10-gingerol suppressed osteoclast markers in RAW264.7 cells [osteoclast-associated immunoglobulin-like receptor, dendrocyte-expressed seven transmembrane protein, and matrix metallopeptidase-9 (Mmp9)] and zebrafish scales [osteoclast-specific cathepsin K (CTSK), mmp2, and mmp9]. Interestingly, nuclear factor of activated T-cells cytoplasmic 1, a master transcription regulator of osteoclast differentiation upstream of the osteoclastic activators, was downregulated in zebrafish scales but showed no alteration in RAW264.7 cells. In addition, 10-gingerol inhibited CTSK activity under cell-free conditions. This is the first study, to our knowledge, that has found that 10-gingerol in GHE could suppress osteoclastic activity in both in vitro and in vivo conditions.
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Sequence Targeting Reagents
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