PUBLICATION

Hoxa2 selectively enhances Meis binding to change a branchial arch ground state

Authors
Amin, S., Donaldson, I.J., Zannino, D.A., Hensman, J., Rattray, M., Losa, M., Spitz, F., Ladam, F., Sagerström, C., Bobola, N.
ID
ZDB-PUB-190719-3
Date
2015
Source
Developmental Cell   32: 265-77 (Journal)
Registered Authors
Sagerström, Charles, Zannino, Denise
Keywords
none
MeSH Terms
  • Animals
  • Branchial Region/metabolism*
  • Cell Line
  • Gene Expression Regulation, Developmental/physiology*
  • Homeodomain Proteins/metabolism*
  • Mice
  • Myeloid Ecotropic Viral Integration Site 1 Protein
  • Neoplasm Proteins/metabolism
  • Transcription Factors/metabolism
(all 9)
PubMed
25640223 Full text @ Dev. Cell
Abstract
Hox transcription factors (TFs) are essential for vertebrate development, but how these evolutionary conserved proteins function in vivo remains unclear. Because Hox proteins have notoriously low binding specificity, they are believed to bind with cofactors, mainly homeodomain TFs Pbx and Meis, to select their specific targets. We mapped binding of Meis, Pbx, and Hoxa2 in the branchial arches, a series of segments in the developing vertebrate head. Meis occupancy is largely similar in Hox-positive and -negative arches. Hoxa2, which specifies second arch (IIBA) identity, recognizes a subset of Meis prebound sites that contain Hox motifs. Importantly, at these sites Meis binding is strongly increased. This enhanced Meis binding coincides with active enhancers, which are linked to genes highly expressed in the IIBA and regulated by Hoxa2. These findings show that Hoxa2 operates as a tissue-specific cofactor, enhancing Meis binding to specific sites that provide the IIBA with its anatomical identity.
Genes / Markers
Marker Marker Type Name
dlx2aGENEdistal-less homeobox 2a
hoxa2bGENEhomeobox A2b
meis1aGENEMeis homeobox 1 a
meis1bGENEMeis homeobox 1 b
meis2aGENEMeis homeobox 2a
meis3GENEmyeloid ecotropic viral integration site 3
1 - 6 of 6
Show
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
meis1aMO3-meis1aMRPHLNO
meis1bMO6-meis1bMRPHLNO
meis2aMO4-meis2aMRPHLNO
meis3MO6-meis3MRPHLNO
1 - 4 of 4
Show
Fish
No data available
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Amin, S., Donaldson, I.J., Zannino, D.A., Hensman, J., Rattray, M., Losa, M., Spitz, F., Ladam, F., Sagerström, C., Bobola, N. (2015) Hoxa2 selectively enhances Meis binding to change a branchial arch ground state. Developmental Cell. 32:265-77.