PUBLICATION
Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish CNS
- Authors
- Kim, E., Jeong, I., Chung, A.Y., Kim, S., Kwon, S.H., Seong, J.Y., Park, H.C.
- ID
- ZDB-PUB-190326-3
- Date
- 2019
- Source
- Scientific Reports 9: 5025 (Journal)
- Registered Authors
- Chung, Ah-Young, Jeong, Inyoung, Kim, Eunmi, Kim, Suhyun, Park, Hae-Chul
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Brain/metabolism*
- Gene Expression
- In Situ Hybridization
- In Situ Hybridization, Fluorescence
- Larva/genetics
- Larva/metabolism
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Mesencephalon/metabolism
- Nerve Net/cytology
- Nerve Net/metabolism*
- Neurons/metabolism*
- Neuropeptides/genetics*
- Neuropeptides/metabolism
- Preoptic Area/metabolism
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Rhombencephalon/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30903017 Full text @ Sci. Rep.
Citation
Kim, E., Jeong, I., Chung, A.Y., Kim, S., Kwon, S.H., Seong, J.Y., Park, H.C. (2019) Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish CNS. Scientific Reports. 9:5025.
Abstract
Spexin (SPX) is a highly conserved neuropeptide that is widely expressed in mammalian brain and peripheral tissue. In teleost, SPX1 is mainly expressed in the brain and ovary, and is involved in reproduction and food intake. A second form of SPX, SPX2, was recently identified in chick, Xenopus, and zebrafish. The expression pattern and roles of SPX2 are unknown. SPX (spx1) is highly expressed in the vertebrate brain, but its distribution, circuits, and interactions with its putative receptor are unknown. Here, we observed expression of spx1 in the midbrain and hindbrain, and spx2 in the hypothalamic preoptic area using in situ RNA hybridization in zebrafish. Analysis of transgenic reporter zebrafish revealed that hindbrain SPX1 neurons are PAX2+ inhibitory interneurons and project to the spinal cord, where they interact with galanin receptor 2b (GALR2b) neurons, suggesting that hindbrain SPX1 neurons are reticulospinal neurons. spx1 mRNA and SPX1 reporter expression were observed in dorsal habenula (dHb). SPX1 neurons in the dHb project to the interpeduncular nucleus (IPN), where GALR2a and GALR2b expression was also observed, suggesting that habenula SPX1 neurons may interact with GALR2a/2b in the IPN.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping