PUBLICATION

Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate

Authors
Araya, C., Häkkinen, H.M., Carcamo, L., Cerda, M., Savy, T., Rookyard, C., Peyriéras, N., Clarke, J.D.W.
ID
ZDB-PUB-190214-5
Date
2019
Source
Scientific Reports   9: 1835 (Journal)
Registered Authors
Araya Garcia, Claudio, Clarke, Jon, Peyriéras, Nadine, Savy, Thierry
Keywords
none
MeSH Terms
  • Actins/metabolism
  • Animals
  • Animals, Genetically Modified
  • Body Patterning
  • Cadherins/metabolism*
  • Cell Membrane/metabolism
  • Cell Movement
  • Epithelium/embryology
  • Gene Expression Regulation*
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/metabolism
  • Heterocyclic Compounds, 4 or More Rings/pharmacology
  • Microscopy, Confocal
  • Morphogenesis
  • Myosin Type II/metabolism*
  • Myosins/metabolism
  • Neural Crest/embryology*
  • Neural Plate/embryology
  • Oligonucleotides/pharmacology
  • Prospective Studies
  • Zebrafish/embryology
  • Zebrafish Proteins/metabolism*
PubMed
30755665 Full text @ Sci. Rep.
Abstract
Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation - the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.
Errata / Notes
corrected by ZDB-PUB-190518-6
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping