PUBLICATION
Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate
- Authors
- Araya, C., Häkkinen, H.M., Carcamo, L., Cerda, M., Savy, T., Rookyard, C., Peyriéras, N., Clarke, J.D.W.
- ID
- ZDB-PUB-190214-5
- Date
- 2019
- Source
- Scientific Reports 9: 1835 (Journal)
- Registered Authors
- Araya Garcia, Claudio, Clarke, Jon, Peyriéras, Nadine, Savy, Thierry
- Keywords
- none
- MeSH Terms
-
- Actins/metabolism
- Animals
- Animals, Genetically Modified
- Body Patterning
- Cadherins/metabolism*
- Cell Membrane/metabolism
- Cell Movement
- Epithelium/embryology
- Gene Expression Regulation*
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/metabolism
- Heterocyclic Compounds, 4 or More Rings/pharmacology
- Microscopy, Confocal
- Morphogenesis
- Myosin Type II/metabolism*
- Myosins/metabolism
- Neural Crest/embryology*
- Neural Plate/embryology
- Oligonucleotides/pharmacology
- Prospective Studies
- Zebrafish/embryology
- Zebrafish Proteins/metabolism*
- PubMed
- 30755665 Full text @ Sci. Rep.
Citation
Araya, C., Häkkinen, H.M., Carcamo, L., Cerda, M., Savy, T., Rookyard, C., Peyriéras, N., Clarke, J.D.W. (2019) Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate. Scientific Reports. 9:1835.
Abstract
Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation - the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.
Errata / Notes
corrected by ZDB-PUB-190518-6
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping