PUBLICATION

Effects of circadian clock protein Per1b on zebrafish visual functions

Authors
Nie, K., Wang, K., Huang, D.F., Huang, Y.B., Yin, W., Ren, D.L., Wang, H., Hu, B.
ID
ZDB-PUB-171122-2
Date
2017
Source
Chronobiology International   35(2): 160-168 (Journal)
Registered Authors
Hu, Bing, Wang, Han
Keywords
DA deficiency, Period1b, behavioral contrast sensitivity, zebrafish
MeSH Terms
  • Animals
  • CLOCK Proteins/genetics
  • CLOCK Proteins/metabolism*
  • Circadian Clocks/physiology*
  • Circadian Rhythm/physiology*
  • Dopamine/metabolism
  • Period Circadian Proteins/genetics
  • Period Circadian Proteins/metabolism*
  • Retina/metabolism
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed
29157002 Full text @ Chronobiol. Int.
Abstract
The circadian clock is an endogenous and entrainable time-keeping mechanism with a period of approximately 24 h, operated by transcription/translation feedback loops composed of circadian clock genes and their proteins. The visual system displays robust circadian changes. Relatively little, however, is known about the mechanisms underlying visual circadian rhythmicity. Zebrafish period1b (per1b), as a canonical circadian clock gene, is involved in circadian regulation. Here, we observed that zebrafish per1b mutants exhibit visual defects including reduced behavioral contrast sensitivity and significant retinal dopaminergic deficiency. Further, partially damaged dopaminergic interplexiform cells in wild-type larvae also led to reduced behavioral contrast sensitivity, while exogenous dopamine administration effectively restored the contrast sensitivity of per1b mutants. Taken together, these results suggest that retinal dopaminergic deficiency derived from loss of per1b results in visual defects in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping