PUBLICATION

Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos

Authors
Parolini, M., Bini, L., Magni, S., Rizzo, A., Ghilardi, A., Landi, C., Armini, A., Del Giacco, L., Binelli, A.
ID
ZDB-PUB-171011-9
Date
2018
Source
Environmental pollution (Barking, Essex : 1987)   232: 603-614 (Journal)
Registered Authors
Del Giacco, Luca, Ghilardi, Anna
Keywords
Benzoylecgonine, Cocaine, Ecgonine methyl ester, Illicit drugs, Proteomics, Zebrafish embryos
MeSH Terms
  • Animals
  • Cocaine/analogs & derivatives
  • Cocaine/metabolism
  • Cocaine/toxicity*
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/physiology
  • Fresh Water
  • Illicit Drugs/toxicity*
  • Oxidation-Reduction
  • Oxidative Stress/drug effects
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism*
(all 13)
PubMed
28993024 Full text @ Environ. Pollut.
Abstract
Illicit drugs have been identified as emerging aquatic pollutants because of their widespread presence in freshwaters and potential toxicity towards aquatic organisms. Among illicit drug residues, cocaine (COC) and its main metabolites, namely benzoylecgonine (BE) and ecgonine methyl ester (EME), are commonly detected in freshwaters worldwide at concentration that can induce diverse adverse effects to non-target organisms. However, the information of toxicity and mechanisms of action (MoA) of these drugs, mainly of COC metabolites, to aquatic species is still fragmentary and inadequate. Thus, this study was aimed at investigating the toxicity of two concentrations (0.3 and 1.0 μg/L) of COC, BE and EME similar to those found in aquatic ecosystems on zebrafish (Danio rerio) embryos at 96 h post fertilization through a functional proteomics approach. Exposure to COC and both its metabolites significantly altered the protein profile of zebrafish embryos, modulating the expression of diverse proteins belonging to different functional classes, including cytoskeleton, eye constituents, lipid transport, lipid and energy metabolism, and stress response. Expression of vitellogenins and crystallins was modulated by COC and both its main metabolites, while only BE and EME altered proteins related to lipid and energy metabolism, as well as to oxidative stress response. Our data confirmed the potential toxicity of low concentrations of COC, BE and EME, and helped to shed light on their MoA on an aquatic vertebrate during early developmental period.
Genes / Markers
Marker Marker Type Name
apoa1bGENEapolipoprotein A-Ib
atp5fa1GENEATP synthase F1 subunit alpha
ckbbGENEcreatine kinase, brain b
ckmaGENEcreatine kinase, muscle a
ckmbGENEcreatine kinase, muscle b
cryba1bGENEcrystallin, beta A1b
cryba2aGENEcrystallin, beta A2a
cryba4GENEcrystallin, beta A4
crybb1GENEcrystallin, beta B1
crygm2d1GENEcrystallin, gamma M2d1
1 - 10 of 21
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Expression
Phenotype
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Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
Orthology
Engineered Foreign Genes
No data available
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Citation
Parolini, M., Bini, L., Magni, S., Rizzo, A., Ghilardi, A., Landi, C., Armini, A., Del Giacco, L., Binelli, A. (2018) Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos. Environmental pollution (Barking, Essex : 1987). 232:603-614.