PUBLICATION
Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity
- Authors
- Wan, F., Hu, C.B., Ma, J.X., Gao, K., Xiang, L.X., Shao, J.Z.
- ID
- ZDB-PUB-170126-6
- Date
- 2017
- Source
- Frontiers in immunology 7: 675 (Journal)
- Registered Authors
- Keywords
- adaptive humoral immunity, antigen-presenting cells, identification, mucosal IgZ antibody, origin of T cell subset, zebrafish γδ T cells
- MeSH Terms
- none
- PubMed
- 28119690 Full text @ Front Immunol
Citation
Wan, F., Hu, C.B., Ma, J.X., Gao, K., Xiang, L.X., Shao, J.Z. (2017) Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity. Frontiers in immunology. 7:675.
Abstract
γδ T cells represent an evolutionarily primitive T cell subset characterized by distinct T cell receptors (TCRs) and innate and adaptive immune functions. However, the presence of this T cell subset in ancient vertebrates remains unclear. In this study, γδ T cells from a zebrafish (Danio rerio) model were subjected to molecular and cellular characterizations. The constant regions of zebrafish TCR-γ (DrTRGC) and δ (DrTRDC) were initially identified. Zebrafish γδ T cells accounted for 7.7-20.5% of the total lymphocytes in spleen, head kidney, peripheral blood, skin, gill, and intestine tissues. They possess typical morphological features of lymphocytes with a surface phenotype of γ+δ+CD4-CD8+. Zebrafish γδ T cells functionally showed a potent phagocytic ability to both soluble and particulate antigens. They can also act as an antigen-presenting cell to initiate antigen (KLH)-specific CD4+ TKLH cell activation and to induce B cell proliferation and IgM production. Particularly, zebrafish γδ T cells also play a critical role in antigen-specific IgZ production in intestinal mucus. These findings demonstrated that γδ T cells had been originated as early as teleost fish, which providing valuable insights into the evolutionary history of T cell subset. It is anticipated that this study would be used as a guide to develop a zebrafish model for the cross-species investigation of γδ T cell biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping