PUBLICATION

Retinoic Acid Signaling Regulates Differential Expression of the Tandemly-Duplicated Long Wavelength-Sensitive Cone Opsin Genes in Zebrafish

Authors
Mitchell, D.M., Stevens, C.B., Frey, R.A., Hunter, S.S., Ashino, R., Kawamura, S., Stenkamp, D.L.
ID
ZDB-PUB-150822-1
Date
2015
Source
PLoS Genetics   11: e1005483 (Journal)
Registered Authors
Frey, Ruth, Kawamura, Shoji, Stenkamp, Deborah L., Stevens, Craig
Keywords
Embryos, Retina, Zebrafish, Eyes, Gene expression, Photoreceptors, Microarrays, Gene regulation
Datasets
GEO:GSE63873
MeSH Terms
  • Animals
  • Cell Differentiation
  • Cone Opsins/genetics*
  • Cone Opsins/metabolism
  • Eye/cytology
  • Eye/metabolism
  • Gene Expression Regulation, Developmental
  • Opsins/genetics*
  • Opsins/metabolism
  • Retinal Cone Photoreceptor Cells/physiology*
  • Transcriptome
  • Tretinoin/physiology*
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
(all 15)
PubMed
26296154 Full text @ PLoS Genet.
Abstract
The signaling molecule retinoic acid (RA) regulates rod and cone photoreceptor fate, differentiation, and survival. Here we elucidate the role of RA in differential regulation of the tandemly-duplicated long wavelength-sensitive (LWS) cone opsin genes. Zebrafish embryos were treated with RA from 48 hours post-fertilization (hpf) to 75 hpf, and RNA was isolated from eyes for microarray analysis. ~170 genes showed significantly altered expression, including several transcription factors and components of cellular signaling pathways. Of interest, the LWS1 opsin gene was strongly upregulated by RA. LWS1 is the upstream member of the tandemly duplicated LWS opsin array and is normally not expressed embryonically. Embryos treated with RA 48 hpf to 100 hpf or beyond showed significant reductions in LWS2-expressing cones in favor of LWS1-expressing cones. The LWS reporter line, LWS-PAC(H) provided evidence that individual LWS cones switched from LWS2 to LWS1 expression in response to RA. The RA signaling reporter line, RARE:YFP indicated that increased RA signaling in cones was associated with this opsin switch, and experimental reduction of RA signaling in larvae at the normal time of onset of LWS1 expression significantly inhibited LWS1 expression. A role for endogenous RA signaling in regulating differential expression of the LWS genes in postmitotic cones was further supported by the presence of an RA signaling domain in ventral retina of juvenile zebrafish that coincided with a ventral zone of LWS1 expression. This is the first evidence that an extracellular signal may regulate differential expression of opsin genes in a tandemly duplicated array.
Genes / Markers
Marker Marker Type Name
cyp26b1GENEcytochrome P450, family 26, subfamily b, polypeptide 1
dkk1bGENEdickkopf WNT signaling pathway inhibitor 1b
hoxb6bGENEhomeobox B6b
nr2f5GENEnuclear receptor subfamily 2, group F, member 5
opn1lw1GENEopsin 1 (cone pigments), long-wave-sensitive, 1
opn1lw2GENEopsin 1 (cone pigments), long-wave-sensitive, 2
rhoGENErhodopsin
sfrp1aGENEsecreted frizzled-related protein 1a
socs3aGENEsuppressor of cytokine signaling 3a
wnt11f2GENEwnt 11, family member 2
1 - 10 of 10
Show
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kj15TgTransgenic Insertion
    pd5TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      GFPEFGGFP
      RFPEFGRFP
      1 - 3 of 3
      Show
      Mapping
      No data available
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Mitchell, D.M., Stevens, C.B., Frey, R.A., Hunter, S.S., Ashino, R., Kawamura, S., Stenkamp, D.L. (2015) Retinoic Acid Signaling Regulates Differential Expression of the Tandemly-Duplicated Long Wavelength-Sensitive Cone Opsin Genes in Zebrafish. PLoS Genetics. 11:e1005483.