PUBLICATION
Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs
- Authors
- Yin, L., Maddison, L.A., Li, M., Kara, N., LaFave, M.C., Varshney, G.K., Burgess, S.M., Patton, J.G., Chen, W.
- ID
- ZDB-PUB-150410-5
- Date
- 2015
- Source
- Genetics 200(2): 431-41 (Journal)
- Registered Authors
- Burgess, Shawn, Chen, Wenbiao, Li, Mingyu, Patton, James G., Varshney, Gaurav
- Keywords
- CRISPR/Cas9, conditional mutagenesis, glucose homeostasis, retinal regeneration, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- CRISPR-Cas Systems*
- Gene Expression*
- Gene Order
- Gene Silencing
- Gene Targeting*
- Genetic Vectors/genetics
- Glucose/metabolism
- Hypopigmentation/genetics
- Mutagenesis*
- Phenotype
- RNA, Guide, Kinetoplastida/genetics*
- Transgenes*
- Zebrafish
- PubMed
- 25855067 Full text @ Genetics
Citation
Yin, L., Maddison, L.A., Li, M., Kara, N., LaFave, M.C., Varshney, G.K., Burgess, S.M., Patton, J.G., Chen, W. (2015) Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics. 200(2):431-41.
Abstract
Determining the mechanism of gene function is greatly enhanced using conditional mutagenesis. However, generating engineered conditional alleles is inefficient and has only been widely used in mice. Importantly, multiplex conditional mutagenesis requires extensive breeding. Here we demonstrate a system for one-generation multiplex conditional mutagenesis in zebrafish (Danio rerio) using transgenic expression of both cas9 and multiple sgRNAs. We describe five distinct zebrafish U6 promoters for sgRNA expression and demonstrate efficient multiplex biallelic inactivation of tyrosinase, insulin receptor a and b, resulting in defects in pigmentation and glucose homeostasis. Furthermore, we demonstrate temporal and tissue-specific mutagenesis using transgenic expression of Cas9. Heat-shock inducible expression of cas9 allows temporal control of tyr mutagenesis. Liver-specific expression of cas9 disrupts insulin receptor a and b, causing fasting hypoglycaemia and postprandial hyperglycemia. We also show that delivery of sgRNAs targeting ascl1a into the eye leads to impaired damage-induced photoreceptor regeneration. Our findings suggest that CRISPR/Cas9-based conditional mutagenesis in zebrafish is not only feasible but rapid and straightforward.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping