PUBLICATION

The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation

Authors
Abramsson, A., Kettunen, P., Banote, R.K., Lott, E., Li, M., Arner, A., and Zetterberg, H.
ID
ZDB-PUB-130722-16
Date
2013
Source
Developmental Biology   381(2): 377-88 (Journal)
Registered Authors
Kettunen, Petronella, Zetterberg, Henrik
Keywords
amyloid, Appb, motor neuron, development, zebrafish, locomotion
MeSH Terms
  • Amyloid beta-Protein Precursor/genetics
  • Amyloid beta-Protein Precursor/metabolism*
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • Behavior, Animal
  • Body Patterning
  • Electrical Synapses/metabolism*
  • Electrical Synapses/pathology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology
  • Embryonic Development
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Locomotion/physiology
  • Morpholinos/administration & dosage
  • Motor Neurons/metabolism*
  • Motor Neurons/pathology
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/pathology
  • Neurogenesis
  • Swimming
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
23850871 Full text @ Dev. Biol.
Abstract

The amyloid precursor protein (APP) is a transmembrane protein mostly recognized for its association with Alzheimer's disease. The physiological function of APP is still not completely understood much because of the redundancy between genes in the APP family. In this study we have used zebrafish to study the physiological function of the zebrafish APP homologue, appb, during development. We show that appb is expressed in post-mitotic neurons in the spinal cord. Knockdown of appb by 50–60% results in a behavioral phenotype with increased spontaneous coiling and prolonged touch-induced activity. The spinal cord motor neurons in these embryos show defective formation and axonal outgrowth patterning. Reduction in Appb also results in patterning defects and changed density of pre- and post-synapses in the neuromuscular junctions. Together, our data show that development of functional locomotion in zebrafish depends on a critical role of Appb in the patterning of motor neurons and neuromuscular junctions.

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping