PUBLICATION
Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish
- Authors
- Collery, R.F., and Link, B.A.
- ID
- ZDB-PUB-110316-16
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(3): 712-722 (Journal)
- Registered Authors
- Collery, Ross, Link, Brian
- Keywords
- BMP response element, morphogen gradient, organogenesis, in vivo imaging, fluorescent reporter, transgenic zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Bone Morphogenetic Proteins/metabolism*
- Immunohistochemistry
- Microscopy, Fluorescence
- Response Elements/genetics
- Response Elements/physiology
- Signal Transduction/genetics
- Signal Transduction/physiology
- Smad Proteins/metabolism*
- Zebrafish
- PubMed
- 21337469 Full text @ Dev. Dyn.
Citation
Collery, R.F., and Link, B.A. (2011) Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(3):712-722.
Abstract
Bone morphogenic protein (BMP) signaling is fundamental to development, injury response, and homeostasis. We have developed transgenic zebrafish that report Smad-mediated BMP signaling in embryos and adults. These lines express either enhanced green fluorescent protein (eGFP), destabilized eGFP, or destabilized Kusabira Orange 2 (KO2) under the well-characterized BMP Response Element (BRE). These fluorescent proteins were found to be expressed dynamically in regions of known BMP signaling including the developing tail bud, hematopoietic lineage, dorsal eye, brain structures, heart, jaw, fins, and somites, as well as other tissues. Responsiveness to changes in BMP signaling was confirmed by observing fluorescence after activation in an hsp70:bmp2b transgenic background or by inhibition in an hsp70:nog3 background. We further demonstrated faithful reportage by the BRE transgenic lines following chemical repression of BMP signaling using an inhibitor of BMP receptor activity, dorsomorphin. Overall, these lines will serve as valuable tools to explore the mechanisms and regulation of BMP signal during embryogenesis, in tissue maintenance, and during disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping