PUBLICATION
Zebrafish aplnra functions in epiboly
- Authors
- Nornes, S., Tucker, B., and Lardelli, M.
- ID
- ZDB-PUB-091120-66
- Date
- 2009
- Source
- BMC research notes 2: 231 (Journal)
- Registered Authors
- Lardelli, Michael, Nornes, Svanhild, Tucker, Ben
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 19922670 Full text @ BMC Res. Notes
Abstract
BACKGROUND: The zebrafish, Danio rerio, possesses the paralogous genes aplnra and aplnrb that are duplicates of an ancestral orthologue of the human APLNR gene encoding a G-protein coupled receptor that binds the peptide ligand APELIN and is required for normal cardiovascular function. aplnrb is required for migration of cells contributing to heart development in zebrafish embryos. aplnra is transcribed in a complex pattern during early development but its function in embryogenesis is largely unknown. FINDINGS: Blockage of translation of aplnra mRNA in zebrafish embryos results in retarded or failed epiboly with the blastoderm apparently disconnected from the nuclei of the yolk syncytial layer. Gastrulation is also defective. Failure of correct tail extension is observed with ectopic structures resembling somites positioned dorsal to the spinal cord. CONCLUSIONS: aplnra, unlike its duplicate aplnrb, is essential for normal epiboly, although this function appears to be independent of signalling activated by zebrafish Apelin. The defects in epiboly caused by loss of aplnra activity appear, at least partially, to be due to a requirement for aplnra activity in the yolk syncytial layer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping