PUBLICATION

Hedgehog signalling is required for correct anteroposterior patterning of the zebrafish otic vesicle

Authors
Hammond, K.L., Loynes, H.E., Folarin, A.A., Smith, J., and Whitfield, T.T.
ID
ZDB-PUB-030304-16
Date
2003
Source
Development (Cambridge, England)   130(7): 1403-1417 (Journal)
Registered Authors
Hammond, Katherine L., Loynes, Helen, Smith, Lucy, Whitfield, Tanya T.
Keywords
Hedgehog, Sonic hedgehog, Tiggy-winkle hedgehog, Inner ear, Zebrafish, Mirror image duplication, slow muscle omitted, chameleon, Otic vesicle, Axis formation
MeSH Terms
  • Animals
  • Ear/abnormalities
  • Ear/embryology*
  • Embryonic Induction/physiology*
  • Ganglia/embryology
  • Hedgehog Proteins
  • Receptors, Cell Surface/metabolism
  • Trans-Activators/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins
(all 10)
PubMed
12588855 Full text @ Development
Abstract
Currently, few factors have been identified that provide the inductive signals necessary to transform the simple otic placode into the complex asymmetric structure of the adult vertebrate inner ear. We provide evidence that Hedgehog signalling from ventral midline structures acts directly on the zebrafish otic vesicle to induce posterior otic identity. We demonstrate that two strong Hedgehog pathway mutants, chameleon (con(tf18b)) and slow muscle omitted (smu(b641)) exhibit a striking partial mirror image duplication of anterior otic structures, concomitant with a loss of posterior otic domains. These effects can be phenocopied by overexpression of patched1 mRNA to reduce Hedgehog signalling. Ectopic activation of the Hedgehog pathway, by injection of sonic hedgehog or dominant-negative protein kinase A RNA, has the reverse effect: ears lose anterior otic structures and show a mirror image duplication of posterior regions. By using double mutants and antisense morpholino analysis, we also show that both Sonic hedgehog and Tiggy-winkle hedgehog are involved in anteroposterior patterning of the zebrafish otic vesicle.
Genes / Markers
Marker Marker Type Name
disp1GENEdispatched homolog 1 (Drosophila)
dlx3bGENEdistal-less homeobox 3b
dzip1GENEDAZ interacting zinc finger protein 1
eya1GENEEYA transcriptional coactivator and phosphatase 1
fgf8aGENEfibroblast growth factor 8a
fstaGENEfollistatin a
gli1GENEGLI family zinc finger 1
gli2aGENEGLI family zinc finger 2a
hmx3aGENEH6 family homeobox 3a
ihhbGENEIndian hedgehog signaling molecule b
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Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
Df(Chr07)t4/t4standard conditionsProtruding-mouth
Df(Chr07)t4/t4standard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO2-shhastandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO2-shhastandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO3-shhbstandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO3-shhbstandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO3-shhbstandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO3-shhbstandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO3-shhbstandard conditionsProtruding-mouth
Df(Chr07)t4/t4 + MO3-shhbstandard conditionsProtruding-mouth
1 - 10 of 38
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    t4
      		Deficiency
      tc41
        		Unknown
        tf18b
          		Point Mutation
          tf219
            		Point Mutation
            tk241
              		Point Mutation
              tm229
                		Point Mutation
                ts269
                  		Point Mutation
                  ts294e
                    		Point Mutation
                    ty97
                      		Point Mutation
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      shhaMO2-shhaMRPHLNO
                      shhbMO3-shhbMRPHLNO
                      1 - 2 of 2
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                      Fish
                      Fish
                      Df(Chr07)t4/t4
                      Df(Chr07)t4/t4
                      Df(Chr07)t4/t4 + MO2-shha
                      Df(Chr07)t4/t4 + MO3-shhb
                      disp1tf18b/tf18b
                      dzip1ts294e/ts294e
                      gli1ts269/ts269
                      ndr2tf219/tf219
                      ndr2tf219/tf219; Df(Chr07)t4/t4
                      nototk241/tk241
                      1 - 10 of 16
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                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      No data available
                      Mapping
                      No data available
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                      Citation
                      Hammond, K.L., Loynes, H.E., Folarin, A.A., Smith, J., and Whitfield, T.T. (2003) Hedgehog signalling is required for correct anteroposterior patterning of the zebrafish otic vesicle. Development (Cambridge, England). 130(7):1403-1417.