PUBLICATION
Dicistronic gene expression in developing zebrafish
- Authors
- Fahrenkrug, S.C., Clark, K., Dahlquist, M.O., and Hackett, P.B.
- ID
- ZDB-PUB-020805-2
- Date
- 1999
- Source
- Marine biotechnology (New York, N.Y.) 1(6): 552-561 (Journal)
- Registered Authors
- Clark, Karl, Fahrenkrug, Scott C., Hackett, Perry B.
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 10612680 Full text @ Mar. Biotechnol.
Citation
Fahrenkrug, S.C., Clark, K., Dahlquist, M.O., and Hackett, P.B. (1999) Dicistronic gene expression in developing zebrafish. Marine biotechnology (New York, N.Y.). 1(6):552-561.
Abstract
Internal ribosome entry sites (IRESs) allow ribosomal access to messenger RNA without a requirement for cap recognition and subsequent scanning to an initiator AUG. Hence, IRESs have been adapted into dicistronic vectors for the expression of more than one gene from a single mRNA. Dicistronic vectors have been used for many applications in mammalian tissue culture and transgenesis. However, whether the IRESs from mammalian viruses function without temporal or spatial restrictions in nonmammalian organisms like zebra fish (Danio rerio) is unknown. Therefore, we have examined the expression capabilities of the encephalomyocarditis virus (EMCV) IRES during zebrafish embryogenesis. We determined that the EMCV IRES was sufficient to permit detectable expression of several second cistron reporters during zebrafish embryogenesis, including luciferase and green fluorescent protein. This suggests that our dicistronic vectors are suitable for general use in any vertebrate system, from fish to humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping