PUBLICATION
Structural, biochemical, and expression analysis of two distinct insulin-like growth factor I receptors and their ligands in zebrafish
- Authors
- Maures, T., Chan, S.-J., Xu, B., Sun, H., Ding, J., and Duan, C.
- ID
- ZDB-PUB-020429-3
- Date
- 2002
- Source
- Endocrinology 143(5): 1858-1871 (Journal)
- Registered Authors
- Chan, Shu Jin, Duan, Cunming
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- DNA Primers
- DNA, Complementary/biosynthesis
- DNA, Complementary/genetics
- Gene Expression Regulation, Developmental/genetics*
- Growth/genetics*
- Growth/physiology
- Immunohistochemistry
- In Situ Hybridization
- Insulin-Like Growth Factor I/biosynthesis
- Insulin-Like Growth Factor I/genetics
- Insulin-Like Growth Factor II/biosynthesis
- Insulin-Like Growth Factor II/genetics
- Larva/metabolism
- Ligands
- Molecular Sequence Data
- Peptides/chemical synthesis
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Receptor, IGF Type 1/biosynthesis*
- Receptor, IGF Type 1/chemistry
- Receptor, IGF Type 1/genetics*
- Receptor, Insulin/biosynthesis
- Receptor, Insulin/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Somatomedins/biosynthesis
- Somatomedins/genetics
- Tissue Distribution
- Zebrafish
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics
- PubMed
- 11956169 Full text @ Endocrinology
Citation
Maures, T., Chan, S.-J., Xu, B., Sun, H., Ding, J., and Duan, C. (2002) Structural, biochemical, and expression analysis of two distinct insulin-like growth factor I receptors and their ligands in zebrafish. Endocrinology. 143(5):1858-1871.
Abstract
We have cloned and characterized cDNAs encoding the zebrafish IGF ligands and receptors. Sequence comparison showed that the primary structures of zebrafish IGF-I, IGF-II, and IGF-I receptors (IGF-IRs) have been highly conserved in vertebrates. In contrast to the presence of a single IGF-IR gene in mammals, two distinct IGF-IR genes, termed igf-1ra and igf-1rb, were found in zebrafish. Structural and phylogenetic analyses indicated that both genes are orthologous to the human igf-1r gene. Immunoprecipitation studies with specific antibodies showed that both IGF-IR genes are expressed and both receptors bind to IGFs and des(1-3)IGF-I, but not to insulin. The spatio-temporal expression patterns of the two IGF-IRs and their ligands were determined using a combination of RT-PCR, whole mount in situ hybridization, and immunocytochemistry. Transcripts for both IGF-I and -II mRNAs were found throughout embryogenesis in a ubiquitous manner. In adult tissues, IGF-I mRNA was more abundant in liver and testis, and its level was increased after GH treatment, whereas IGF-II mRNA was not regulated by GH. IGF-IRa and IGF-IRb mRNAs and proteins were expressed in overlapping spatial domains, but exhibited distinct temporal expression patterns. In particular, the relative level of IGF-IRa mRNA was low during early embryogenesis and increased in the hatched larva, whereas the situation was reversed for IGF-IRb mRNA. In adult zebrafish, the overall tissue distribution patterns of the two IGF-IRs were similar, but there were differences in their cellular localization and relative abundance in defined cells/regions. The differential expression pattern of IGF-IRa and IGF-IRb suggest that they may play distinct roles in regulating the growth and development of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping