Figure 4—figure supplement 2.

Figure 4—figure supplement 2. Ectopic integration of Sema7A^sec distant from the neuromast induces sensory neurite extension.

(A) Micrographs of a section of the lateral-line nerve between two neuromasts from a 3-dpf heat-shocked larva depicts a few extended neurites (magenta) reaching toward the cells expressing the ectopic Sema7A^sec (orange). The Sema7A^sec integration site with the sensory axonal projections was imaged for one hour to visualize axonal dynamics. Micrographs (i-iii) at three distinct time-points from the time-lapse video microscopy show directed branching (cyan arrowheads) of the sensory neurites from the lateral-line nerve toward the ectopic Sema7A^sec sources. Each panel depicts the ectopic integration site and the associated sensory neurites from three different planes, XY, XZ, and YZ. White arrowheads in XZ and YZ planes highlight the close association of the sensory axons with the ectopic Sema7A^sec sources. Melanocytes (yellow arrowheads) along the horizontal myoseptum intermittently block the visibility of the lateral-line nerve. Scale bar, 20 µm.