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Fig. 5 Analysis of spontaneous eye movements in knockout (KO) zebrafish. (A) Representative image of spontaneous eye movement in wild-type (WT) and KO zebrafish at 5 dpf. (B) Individual representation of spontaneous eye movement in WT and srpk3 KO siblings. Red lines represent right eye and blue lines represent left eye of 5 dpf zebrafish larvae. (C) Eye movement frequency in the left (L) and right (R) eye of srpk3 KO zebrafish when compared to its WT siblings. (D) Ocular angle significantly decreases in both left and right eye in KO zebrafish, compared to WT siblings. (E) srpk3 KO zebrafish take significant longer time to reset in slow phase when compared to their WT siblings during spontaneous eye movement. Video recording of WT (n = 11) and KO (n = 12) zebrafish for 3 min. All data is represented as mean ± SEM. **p < 0.01, ***p < 0.001 by t test.