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Fig. 4 Generation of srpk3 zebrafish knockout (KO) line. (A) Disruption of protein domains in KO zebrafish while wild-type (WT) zebrafish show intact srpk3 protein. Protein kinase domain 1, PK-1; protein kinase domain 2, PK-2. (B) KO zebrafish fail to develop swim bladder inflation at 5 dpf when compared to WT siblings (red arrow). (C) Expression analysis of neuronal markers (her4, th, islet1) and srpk3 at 72hpf. Scale bars; 200 μm. (D, E) Reduced expression level of srpk3 mRNA in KO zebrafish. Three adult brains for each group were used in qRT-PCR experiments (n = 3 for WT and n = 3 for KO).
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Full text @ Ann. Neurol.