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Fig. 5 Severe ciliary disorder in zebrafish cfap53; mns1 double mutants. (A) Schematic of the zebrafish cfap53 locus and targeting of exon 2 with guide RNA. (B) Nucleotide sequence of the cfap53 mutant allele compared with wild type. (C) Schematic and amino acid sequence of the predicted Cfap53 mutant protein compared with wild type. (D) Percentage of embryos showing a heart looping defect (48 hpf) compared with wild type (three technical replicates). Data are presented as mean±s.d. *P≤0.05 (two-tailed Student's t-test). (E) Wild-type (WT) zebrafish embryo morphology (24 hpf). (F) A cfap53−/−; mns1−/− double mutant embryo with curved body axis (24 hpf). Scale bars: 1 mm.