Fig. 2 MNS1 localisation to motile cilia in zebrafish and mouse tissues. (A) Immunofluorescence with antibodies to HA epitope (green) and acetylated tubulin (magenta) of cilia in zebrafish KV [10-somite stage (14 hpf)]. (B) Immunofluorescence with antibodies to HA (green) and acetylated tubulin (magenta) of zebrafish pronephric duct (PD) cilia (24 hpf). (C) Immunofluorescence with antibodies to MNS1 (green) and acetylated tubulin (magenta) of E8.0 mouse node cilia. (D) Immunofluorescence with antibodies to MNS1 (green) and γ tubulin (magenta) of E8.0 mouse node cilia. (E) Immunofluorescence with antibodies to MNS1 (green) and acetylated tubulin (magenta) of adult mouse trachea. Insets in C-E show higher magnification views. Scale bars: 5 µm (A,B,E, main panel); 2 µm (C,D, main panels); 10 µm (C-E, insets).
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