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Fig. 3 axin2gh1/gh1;nkd1gh2/gh2 mutants are sensitive to activation at the destruction complex level. (A) Embryos were treated with varying concentrations of BIO from 4 – 30 hpf with representative images taken of the wild type embryos. (B) In wild type embryos, the area of the eye has a decreasing linear association with the concentration of BIO with all embryos being eyeless at 1.5 µM. In contrast, the majority of the axin2gh1/gh1;nkd1gh2/gh2 mutant embryos were eyeless at 0.5 µM of BIO. (C) The axin2gh1/gh1;nkd1gh2/gh2 mutant has no change in the size of eye in the control treatment but has significantly smaller eyes at 0.5 µM of BIO when compared with wild type revealing that the axin2gh1/gh1;nkd1gh2/gh2 mutants are sensitive to BIO. Measurements were performed on imageJ and a t test was used with ** = p value < 0.01. All error bars represent SEM. No statistics were performed on the axin2gh1/gh1 and nkd1gh2/gh2 single mutants.