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Fig. 5 Tie1 signaling regulates gene expression involved in lymphatic development. (A) Schematic of the workflow. RNA-seq was performed in tie1−/− embryos and their WT and tie1+/− siblings (tie1+/?) (n=2 RNA samples each). (B) Volcano plot of the tie1−/− and tie1+/? gene set. The red, green and black dots represent upregulated (in tie1−/− embryos), downregulated and non-significant genes, respectively, with fold change >1 and P<0.05. (C) MA plot of 1942 DEGs (blue dots) with q-value <0.1 in the tie1−/− and tie1+/? gene set. (D) Heatmap of the most significant DEGs using log2(TPM+1) values. (E) Representative GO biological processes of the DEGs. (F) Heatmap of the DEGs associated with the GO terms ‘Vascular development’, ‘Migration’ and ‘Cell cycle’. Genes are ranked by log2 fold change tie1−/−/tie1+/?. (G) Heatmap of the DEGs associated with the FOXO1 and MYC signaling. Genes are ranked by log2 fold change tie1−/−/tie1+/?.