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Fig. 1 Generation of zebrafish Stat3 mutants. Schematic of the STAT3 protein outlining the various functional domains: N-terminal, CCD (coiled-coil domain), DBD (DNA binding domain), linker, SH2 (Src homology 2 domain), and TAD (transactivation domain), along with relevant sections of the zebrafish stat3 gene showing introns (black lines) and exons (colored boxes) encoding the indicated protein domains as well its targeting by sgRNAs (a). Sequence traces of representative homozygous wild-type (WT, stat3wt/wt) and knockout (KO, stat3ko/ko) (b) and WT and transactivation domain truncation (ΔTAD, stat3Δtad/Δtad) (c) mutant fish, with nucleotides and corresponding amino acids shown. In each case, nucleotide sequences deleted are boxed in purple with de novo amino acids in red. Schematic of Stat3 proteins expressed in WT, KO, and ΔTAD fish (d).