Fig. 1

Fig. 1 High-resolution, high-speed tracking of zebrafish behavior in a large environment.

A Schematic illustration of our experimental setup and analysis pipeline. Fish swam in an arena where visual stimuli were projected underneath. We acquired high-resolution images at a speed of 290 Hz across the arena of up to 90 mm in diameter. Data analysis pipelines based on a deep neural network identified the fish loci and body postures. We measured spontaneous exploration while the visual stimuli are stopped (Spont.), and visually-induced optomotor response (OMR) while the visual stimuli are moving. See Fig. S1A and the “Methods” section for details. B Head centroid trajectories in a small, walled environment (30 mm) and a large, unwalled environment (90 mm). Each swim episode is colored in either light green or black to separate different swim episodes visually. C Distributions of head centroids during experiments across tested fish. N = 22 and N = 20 fish for the small and large dishes, respectively. D Swimming distance per minute during spontaneous exploration (red) and optomotor response (OMR, black). Transparent circles represent individual fish. **p = 0.0081; ***p = 1.7 × 10⁻⁶ from 2-sample t-test. Error bars represent standard deviations across tested fish. E Expanded head centroid trajectories from the outlined central parts of the small arena (left) and the large arena (right) from (B). The large environment facilitates straight swim patterns.