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Fig. 6 The size of telencephalon in HV and HVP embryos at 24, 30 and 48 hpf. (A) WM Chromogenic ISH showing foxg1 mRNA expression in HV and HVP at specific stages. Black arrows: telencephalon. (B) 3D volumetric comparison of telencephalon size (calculated using length, depth and width measurements from data in A; see also Fig. S8A-E) in HV versus HVP from 24 to 48 hpf. Bars indicate mean±s.d., dots indicate individual embryos. ns (1): P=0.1104; ns (2): P>0.99. (C) Comparison of the changes in telencephalic volume over time. Spots and bars indicate the mean±s.d. ns: P>0.99. (D) Representative examples of in situ hybridization chain reaction (HCR) for foxg1 mRNA observed in HV versus HVP embryos at 24, 30 and 48 hpf. (E) Volume measurements of telencephalon region marked by foxg1 from data in D observed in HV and HVP embryos at different stages. Bars indicate median and 95% CI, dots indicate individual embryos. ns: P>0.99. (F) Comparison of telencephalon volume growth rates in HV and HVP embryos (data from E). Spots indicate mean, bars indicate s.d. ns (1): P>0.99; ns (2): P=0.6064. *P≤0.05; **P≤0.01; ****P≤0.0001 (statistical test details found in Table S6). ns, not significant. Scale bars: 150 µm (A); 50 µm (D).