ZFIN Image: Breuer et al., 2024, Fig. 3

Image

Figure Caption

Fig. 3 Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast (runx2a, runx2b, sp7/osterix, bglap and postna) and osteoclast related genes (ctsk) screened at 9 dpf and 12 dpf in chd7+/+ and chd7-/-larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7+/+ (n = 5) and chd7-/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7+/+ and n = 8 for chd7-/-). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7+/+ (n = 5) and chd7-/- (n = 5) larvae. Scale bar = 60 μm. (F) TRAP staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. *p < 0.05; **p < 0.01 ***p < 0.001; ***p < 0.0001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Bone Miner. Res.