ZFIN Image: Moreno-Campos et al., 2024, Fig 1

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Figure Caption

Fig 1 construction of an F0 CRISPR screen for ENS development.

(A) tSNE plot shows five distinct sub-clusters after the subset analysis and re-clustering of Clusters 5 and 12 from the [20] 68–70 hpf data set. (B) Violin plots reveal high single-cell expression distribution in sub-cluster 3 of ENS candidate genes. (C) Twelve genes underwent a comprehensive CRISPR screen, involving bioinformatic design, and CRISPR-Cas9 mutagenesis in -8.3phox2bb:Kaede zebrafish larvae to visualize enteric cells. The screening strategy included subsequent genotyping validation and high-content phenotyping.

Acknowledgments

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