ZFIN Image: Rutherford et al., 2024, Fig. 4.

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Figure Caption

Fig. 4.

RNA sequencing reveals that microglia replacement rescues antiviral immune response in 4 wpf rnaset2 mutants. (A and B) Volcano plot of differentially expressed genes between rnaset2 sham versus WT sham (A) and rnaset2 transplanted versus rnaset2 sham groups (B). Significantly differentially regulated genes are shown in red. The top 25 differentially expressed genes are annotated. (C) Gene ontology (GO) plot showing significantly downregulated pathways in rnaset2 microglia-depleted transplanted animals compared with microglia-depleted sham controls, identified by gProfiler. Pathways relating to immune and antiviral response are indicated in bold. (D) Gene set enrichment analysis (GSEA) in rnaset2 microglia-depleted transplanted animals compared with rnaset2 microglia-depleted sham controls. (E and F) Heatmap of genes belonging to response to virus (E) and ISG15 antiviral mechanism (F) GO pathways for all genes significantly up-regulated in rnaset2 sham animals. Fold change relative to WT sham is indicated by color, with red indicating higher expression. All data shown correspond to microglia-depleted animals. See also SI Appendix, Fig. S4.

Acknowledgments

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