Macrophage response after PEG–PAMAM–Rho injection. Macrophage area in each animal (a), rostral area (b) and caudal area (c). (d) Representative images of macrophages (mpeg1:GFP, green) in the larvae (brightfield) at 48 hpi and 72 hpi: left: merged, middle: mpeg1:EGFP (green), right: animal (brightfield). Scale bar: 500 µm. Data are presented in box and whisker plots with minimum to maximum, and the points represent the data of each individual animal (n = 13–48; details in Table S1). Data were logarithmically transformed to fit a normal Gaussian distribution. Statistical analysis was performed within timepoints to compare different conditions using two-way ANOVA followed by Tukey’s multiple comparisons test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
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