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Fig. 5 Comparative analysis of the transcriptomic profiles of zebrafish and healthy human epicardial adipocytes (A) Schematic of the cross-species analysis focusing on high confidence “one-to-one” orthologs between zebrafish and human genes. (B) PCA score plot showing clustering of adipocyte populations according to species and tissue type. (C) Heatmap of the top 34 differentially expressed genes (DEGs) in the eAT compared with classic white fat depots, revealing a shared transcriptomic signature of the eAT across species. Shared upregulated or downregulated DEGs in zebrafish and human eATs are highlighted in black boxes. (D) Scatterplot displaying commonly upregulated (red box) and downregulated (green box) genes in human and zebrafish eAT as well as highly expressed genes in human eAT that are downregulated in the zebrafish tissue (orange box) and vice versa (blue box). (E) Top enriched pathway (KEGG and Reactome) of the 197 upregulated genes in zebrafish and human eAT compared with visceral (vAT) and subcutaneous (SAT) fat, respectively. (F) KEGG pathway and Reactome analyses of the 216 downregulated genes in zebrafish vAT and human SAT compared with eAT. Data in (D) are expressed as log2 fold change. Data in (E) and (F) are expressed as −log[P] adjusted for multiple comparisons. Data were obtained from three zebrafish eATs and vATs and four human eATs and sATs.