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Fig. 3 Overexpression of Pcgf5 gene promotes the neural induction process in P19 cells. (A-C) qPCR and Western Blot was used to analyse the overexpression level of Pcgf5. Full-length gels before cropping are noted in Fig. S6. (D,E) Representative images show immunofluorescence of Sox2 and Oct4 from WT and Pcgf5-OE without RA induction. Scale bar, 5 μm. (F,G) Quantification of (D) and (E). More than 100 cells were counted in each experiment. (H,I) Following the overexpression of Pcgf5, qPCR was utilized to evaluate the expression of Pax6 and Oct4. (J,K) Western Blot was used to detect the expression of Sox2 and Oct4 after Pcgf5 overexpression. Full-length gels before cropping are noted in Fig. S6. Data are presented as means ± SEM of at least three independent experiments (A,C,H -I,K), * (p < 0.05), ** (p < 0.01),*** (p < 0.001), **** (p < 0.0001), ns: no significance.