ZFIN Image: Guo et al., 2024, Figure 6.

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Figure Caption

Figure 6.

In vivo transcriptomic analyses in both mbd5^ΔMBD KO and Tg[zhsp70l:FLAGmbd5^iso2-E2AGFP] overexpression larval zebrafish reveal a critical role of Mbd5 in activating genes implicated in ASD. (A) Schematic of transgenic larval collection and statistical analysis of the differentially expressed genes in mbd5^ΔMBD KO and Tg[zhsp70l:FLAGmbd5^iso2-E2AGFP] overexpression samples at 7 dpf at the set threshold. (B) Venn diagram analysis of down-regulated genes in AB vs mbd5^ΔMBD KO and up-regulated genes in Tg(ctrl) vs Tg[zhsp70l:FLAGmbd5^iso2-E2AGFP]. (C) Venn diagram analysis of up-regulated genes in AB vs mbd5^ΔMBD KO and down-regulated genes in Tg(ctrl) vs Tg[zhsp70l:FLAGmbd5^iso2-E2AGFP]. (D–F) GO analysis based on commonly activated genes in (B) reveal significant changes in biological process (D), cellular component (E) and molecular function (F). (G, H) Expression level of genes associated with synaptic development (G) and signaling (H) were validated through qRT-PCR analysis in mbd5^ΔMBD mutants. Each value in (G, H) represents mean ± SD, **P< 0.01, ***P< 0.001, ****P< 0.0001.

Acknowledgments

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