ZFIN Image: Small et al., 2024, Fig. 7

Image

Figure Caption

Fig. 7 Triploid ISVs are not comprised of fewer cells than diploid ISVs in 48 hpf zebrafish embryos. Scale bar in panel A is valid for all panels. A + B) Composite confocal micrographs of diploid (A) and triploid (B) Tg (h2az2a:h2az2a-GFP; kdlr:mCherry) embryos, showing endothelial cell nuclei (yellow arrows). C + D) Cropped, single plane micrographs at Z-planes selected to highlight endothelial cell nuclei in the dorsal (C, 1 arrows), medial (D, 2 arrows), and ventral (D, 3 arrows) planes in a diploid embryo. E-G) Cropped, single plane micrographs at Z-planes selected to highlight endothelial cell nuclei in the dorsal (E), medial (F), and ventral (G) in a triploid embryo. There is no effect of ploidy on the number of cells in the ISVs at 48 hpf (n = 5 embryos per ploidy).

Acknowledgments

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Reprinted from Developmental Biology, 509, Small, C.D., Benfey, T.J., Crawford, B.D., Tissue-specific compensatory mechanisms maintain tissue architecture and body size independent of cell size in polyploid zebrafish, 85-96, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.