ZFIN Image: Small et al., 2024, Fig. 3

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Figure Caption

Fig. 3 Ploidy does not affect myotome size, sarcomere size, or the rate of somitogenesis in zebrafish embryos. A + B) Confocal micrographs of anti-laminin immunostains (magenta channel) co-stained with Draq5 (cyan channel) of diploid (A) and triploid (B) myotomes. Myotome size was not significantly different along the anteroposterior axis or the dorsoventral axis (n = 5 embryos per ploidy, p = 0.18, and p = 0.99 respectively). C + D) DIC micrographs of diploid and triploid myofibers. Sarcomere size was not significantly affected by ploidy (n = 8 embryos per ploidy, p = 1). E) The rate of somitogenesis is not affected by ploidy (n = 8 embryos per ploidy, p = 0.22).

Acknowledgments

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Reprinted from Developmental Biology, 509, Small, C.D., Benfey, T.J., Crawford, B.D., Tissue-specific compensatory mechanisms maintain tissue architecture and body size independent of cell size in polyploid zebrafish, 85-96, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.