|
Fig. 5 Jiuzao polysaccharides mitigate ethanol-induced disturbances and lipid metabolism, reduce oxidative and endoplasmic reticulum stress, and counteract DNA damage in zebrafish larvae. RT-qPCR elucidated the expression patterns of ethanol metabolism-associated genes, cyp2y3 and cyp3a65 (A). RT-qPCR depicted lipid metabolism-associated genes, hmgcra and fasn (B). RT-qPCR of oxidative stress markers, nrf2 and gpxla, was carried out (C). The expression of endoplasmic reticulum stress and DNA damage-associated genes, edem1, chop, and gadd45αa, were assessed (D). Data are expressed as mean ± SD. #<0.05, ##<0.01, ###<0.001 denote significance against the model group as determined by the student's t-test. Different lowercase letters denote significant differences.