Fig. 5

Fig. 5 Hgf/met downregulates E-cadherin by activating MAPK-snail in vivo.

A Increased expression of E-cadherin in the thyroid primordium of hgfa homozygous mutant embryos was rescued by HGF injection. Larvae were in the ventral position, anterior to the right. B The expression of E-cadherin in the thyroid primordium of 5dpf zebrafish larvae under different conditions, including WT larvae treated with U0126 (b), met homozygous mutant embryos rescued by injection of pTol2tg:met (d), and hgfa homozygous mutant embryos rescued by injection of pTol2tg:Snail (f). Larvae were in the ventral position, anterior to the left. C The abnormal thyroid primordium in 5 dpf hgfa homozygous mutant larvae was rescued by specific overexpression of human Snail (pTol2tg:Snail) in thyrocytes. Vehicle con: 5dpf hgfa homozygous mutant larvae injected with pTol2tg:mCherry. D The rescue effects of the actin polymerization inhibitor cytochalasin B (cyto B) on the abnormal thyroid primordium in 5 dpf hgfa homozygous mutant larvae of zebrafish, detected by WISH in 5 dpf larvae. DMSO: 5dpf hgfa homozygous mutant larvae incubated with dimethyl sulfoxide (DMSO). Scale bars: 30 μm for A, B and 100 μm for C, D. Three independent experiments were repeated with similar results.