Figure 6

Figure 6

Safety and efficacy of PKIS hit compounds in in vivo zebrafish embryo models. (A) Zebrafish embryos were dechorionated 24 hours post fertilization (hpf), treated with compounds 30 hpf and scored for their health 120 hpf. Health score 5 = healthy embryos; for each of the following symptoms 1 point was subtracted: cranial malformations, oedema, irresponsiveness, tail curve malformations; health score 0 = death embryos. (B) Dechorionated zebrafish embryos were infected at 28hpf with 1000 CFUs MRSA and treated at 30 hpf. The survival of the embryos was assessed daily by checking their heart beat. (C) Dechorionated zebrafish embryos were treated with serial dilutions of DMSO, GSK2008607A, GW296115X, and GW633459A to assess health parameters (n = 15 for each treatment and concentration). (D) Infected zebrafish embryos were treated with 10 µM GW296115X (n = 40), 10 µM GW633459A (n = 40) or 0.1% DMSO as negative solvent control (n = 80). Uninfected controls were included to assess the health state of the embryos at baseline. The data were collected in two independent experiments. Differences between treated and untreated (i.e., DMSO) groups were tested for statistical significance (*p < 0.05, ***p < 0.001).