Fig 3

Fig 3 Area of the opercle in fish stained with alizarin red at 10 dpf.

(A, B) Live alizarin red stained fluorescent image of a control larva (A) and a larva treated with PFAA+Br (B) (lateral view, anterior to the left). Arrows point at the area of the opercle outlined in the images. The scale bar represents 500 μm. (C) Opercle area measured in control larvae and larvae upon treatment with the indicated mixture (n ≥ 11). Ordinary one-way ANOVA and Dunnett’s multiple comparison test were performed. Significant difference relative to control is indicated: p-value<0.05 (*), p value ≤ 0.005 (**).