FIGURE 1

FIGURE 1

Heartbeat rate analysis and phenotypic characterization of bigheart mutant. (A) Heartbeat rate analysis in wild type (bh^+/+) and bigheart mutant fish (bh^−/−) fish. X-axis represents time in days, and Y-axis represents heartbeat per minute. (B–O) Phenotypic characterization of bigheart mutant. (B–D) represent bright field images of 3 dpf wild type (WT) embryos, heterozygous embryos (bh^+/−), and bigheart homozygous (bh^−/−) mutant embryos, respectively. (E–G) represent respective fluorescence images at 3 dpf. (Images were captured at ×2.5). Yellow arrowheads indicate GFP expression in the eye and red arrowheads indicate location of the heart. (H,J,L,N) represent WT fish at 3 dpf, 15 dpf, 90 dpf and 9 months. (I,K,M,O) represent corresponding images of the bh^−/− fish at 3 dpf, 15 dpf, 90 dpf, and 9 months, respectively (***, p < 0.001, ****, p < 0.0001). The bars indicate the average values ± SD.