ZFIN Image: Aksoy et al., 2024, Figure 2

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Figure Caption

Figure 2

Generating ccnf-deficient zebrafish lines. (A) Design of sgRNA targeting the PEST region of ccnf. (B) +14bp insertion at the CRISPR/Cas9 target site resulting with premature stop codon (C) High resolution melting analysis (HRMA) curves showing WT (ccnf ^+/+), heterozygous (ccnf^+/−) and homozygous (ccnf^−/−) zebrafish lines (D) RFLP assay showing the PCR amplicons of ccnf^+/+, ccnf^+/−and ccnf^−/− digested with AvaII enzyme. (E) Quantification of ccnf^+/− and ccnf^−/− mRNA, relative to ccnf ^+/+ mRNA in 4dpf embryos.

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