Figure 6

Figure 6

Incubating scn1lab and gabra1 crispants with antiepileptic compounds offers protection against seizure-like events. (A): Tracking plots of the different crispants 2 s after the light flashes. Scale bar = 6 mm. The different plots correspond (from left to right) to scrambled, scn1lab crispants treated with DMSO (vehicle), scn1lab crispants treated with two different concentrations of fenfluramine (17.5 µM and 35 µM), topiramate (50 µM and 100 µM) and valproic acid (50 µM and 100 µM). (B–D): Representation of the percentage of larvae classified in the high-activity region. Scrambled treated with DMSO are represented in dark blue in all plots, scn1lab crispants treated with DMSO are represented in light blue in all plots. (B): scn1lab crispants treated with fenfluramine 17.5 µM are represented in light purple; scn1lab crispants treated with fenfluramine 25 µM are represented in dark purple. (C). scn1lab crispants treated with topiramate 50 µM are represented in light pink; scn1lab crispants treated with topiramate 100 µM are represented in dark pink. scn1lab crispants treated with valproic acid 50 µM are represented in light green; scn1lab crispants treated with valproic acid 100 µM are represented in dark green. In all bar graphs, * p < 0.05 (binomial test).