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Fig. 2 Establishment of TAA-induced liver fibrosis model in zebrafish. (A) The Sirius red staining of zebrafish. Figures are magnified at ×100 (n = 8). (B) H&E staining of zebrafish. Figures are magnified at ×100 (n = 8). (C–G) The qPCR analysis of PPARα, FASN, Col1α1b, α-SMA, and TNF-α mRNA expression in zebrafish larvae (n = 3 or 4). The control group consisted of untreated zebrafish at 8 dpf. The mRNA expression was normalized to β-actin mRNA expression and presented as a fold change compared with the control group. ns denotes no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.