Figure 7

Figure 7

Structural integrity and establishment of segment joints are impaired after osterix+ cell ablation

(A) 7 dpa fin regenerates of 3–5 dpa NFP treated runx2:GFP x osterix:NTR zebrafish. Arrowhead, accumulated melanocytes.

(B) Regenerate length and domain sizes (proximal vs. distal to bifurcation, runx2:GFP negative tip region) at 5 dpa after NFP treatment. Welch’s t tests, Mann-Whitney test for tip region. p(§, tip region) = 0.7732, p($, dist. runx2:GFP) = 0.2564, p(#, prox. runx2:GFP) = 0.6341.

(C) Regenerate length and domain sizes at 7 dpa, after 2days recovery. Welch’s t tests. p(§, tip region) = 0.0624, p($, dist. runx2:GFP) = 0.7599, p(#, prox. runx2:GFP) = 0.0100.

(D) 5 dpa fin regenerates of 3–5 dpa DMSO and NFP treated mmp9:GFP x osterix:NTR zebrafish. Black and green arrowheads, segment joints.

(E) 7 dpa fin regenerates of 3–5 dpa DMSO and NFP treated mmp9:GFP x osterix:NTR zebrafish (2 days recovery). Arrowheads, segment joints.

(F) 5 dpa anti-chondroitin sulfate (CS) and anti-Laminin stained runx2:GFP x osterix:NTR fin regenerate sections after NFP treatment. Arrowheads, Laminin and CS signal in non-ablated fins. Red signal in ablated fins, dying osterix+ cells. Scale bars (A, D, E) 200 μm, (F) 50 μm. (B), (C) Mean.