Figure Caption
Figure 4
Mixing of distal blastema cells and osteoblasts
(A) Overview of TEM of regenerate with positions of cells of interest indicated (1–6). Red dashed line, subepithelial basal lamina.
(B) Cells underlying the BLWE with gradual increase of endoplasmic reticulum (er) from distal (1,2) via intermediate (3,4) to proximal positions. More dilated ER in proximal regions with more Golgi complexes (g) and mitochondria (m) suggesting massive protein synthesis. See also Figure S7. epi, epidermis. n, nucleus. Scale bar (A) 50 μm, (B) 2 μm.
(C) UMAP of runx2a.
(D) FACS gates to identify siam:mCherry+, osterix:GFP+, and mCherry/GFP double+ cells at 3 dpa with respective percentages of unlabeled and labeled cell populations.
(E) Single and double ISH of mCherry and gfp transcripts in siam:mCherry x runx2:GFP transgenic zebrafish. Arrowhead, proximal mCherry expression. Red dashed line, amputation plane. Scale bars 100 μm.
(F) Detection of mCherry/GFP protein double+ cells (arrowheads in boxed areas) in 3 dpa fin regenerates of siam:mCherry x runx2:GFP transgenic zebrafish. Scale bar overview 50 μm. Scale bar inset 25 μm.
(G) Quantification of mCherry, GFP double+ cells in siam:mCherry x runx2:GFP, and siam:mCherry x osterix:GFP transgenic zebrafish (experiments shown in [F], [I]). Shadowed interval with arrows, regions outside of transcript detection.
(H) Double ISH of mCherry and gfp transcripts in siam:mCherry x osterix:GFP transgenic zebrafish. Arrowhead, transcript negative region. Red dashed line, amputation plane. Scale bars 100 μm.
(I) Detection of mCherry/GFP protein double+ cells in 3 dpa siam:mCherry x osterix:GFP fin regenerates (arrowheads in boxed areas). Scale bar overview 50 μm. Scale bar inset 25 μm. (G) Mean ± SD.
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