Figure Caption
Figure 3
Lineage tracing of osterix+ and mmp9+ osteoblasts
(A) Experimental design. 4-OHT, 4-hydroxytamoxifen. Flame icon, heat induction. Camera icon, imaging.
(B) Lineage tracing of osterix+ cells. Asterisk, brightness and contrast increased to reveal GFP expression. Scale bar 200 μm.
(C) Fraction of osterix+ and osterix− progeny at 3, 4, and 5 dpa. Kruskal-Wallis.
(D) Density of osterix+ progeny in the 3, 4, and 5 dpa regenerate in a defined square region of interest (fin ray regenerate region close to amputation plane) of 0.028 mm2 (167 μm × 167 μm). One-way Anova (Tukey).
(E) Lineage tracing of mmp9+ cells (joint cells in proximal regenerate [arrowhead], left panel, and cells in distal regenerate, right panel). Scale bar 100 μm. Asterisks indicate autofluorescence. White arrowheads, nGFP+ cells within fin ray region, green arrowheads, nGFP+ cells in interray.
(F) UMAP view of mmp9 expression. Expression is not restricted to joint cells.
(G) Clone size of mmp9+ progeny in the proximal region of the regenerate. One-way Anova (Tukey).
(H) Variable clone size of mmp9+ progeny in the distal region of the regenerate. One-way Anova (Tukey).
(I) Lineage tracing of osterix+ and mmp9+ cells. dsRed2GFP∗, hsp70L:R2nlsGFP x Actb:dsRed2GFP. Scale bar 200 μm.
(J) Magnified view of osterix+ progeny shown in (B) and osterix+/mmp9+ progeny shown in (I). Brackets, distance to the tip of the regenerate. Scale bar 100 μm. (B), (E) EtOH, ethanol vehicle control. (B), (I) Red dashed line, amputation plane. (B), (E), (I) BF, bright field. (C), (D), (G), (H) Mean ± SD.
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