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Fig. 4 Insulin receptors (insra and insrb) are expressed in neurons. Fluorescent in situ hybridization for insra (b) and insrb (f) genes on zebrafish brain sections followed by HuC/D immunohistochemistry (green, c and g) to label neurons and DAPI counterstaining (blue, a and e). (d and h) Merged pictures showed the colocalization of insr (red) with HuC/D immunostaining (green) (see arrows). Note that, other cells in the brain parenchyma (HuC/D-negative) also expressed these insr genes (arrowheads). Scale bar = 50 μm.
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Full text @ J. Comp. Neurol.