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Fig. 4 Downregulation of cebpa rescues the neutrophil lineage expansion of the gfi1aasmu10 mutant. (A,B) Expression of mpx (A) and lyz (B) in embryos of gfi1aasmu10/+ and cebpahkz7/+ outcrossed progeny. WISH of 3 dpf embryos of WT, gfi1aa+/+cebpahkz7/+, gfi1aa+/+cebpahkz7/hkz7, gfi1aasmu10/smu10cebpa+/+, gfi1aasmu10/smu10cebpahkz7/+ and gfi1aasmu10/smu10cebpahkz7/hkz7. Insets show enlargements of the red boxed regions of the CHT. Scale bars: 200 μm. (C,D) Quantification of mpx+ cells (C) and lyz+ cells (D) in each genotype group of mutants. Data are mean±s.d. nmpx=187, nlyz=241.*P<0.05, ****P<0.0001 (one-way ANOVA). ns, not significant (P>0.05). See also Tables S6 and S7.