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Fig. 6 P. aeruginosa membrane vesicles (MVs) production and role of the Prc protease P. aeruginosa PAO1 wild-type strain, the isogenic Δprc mutant, and the Δprc mutant bearing the pBBR/PAPrc plasmid (Table S1) (Δprc + Prc) were grown to exponential phase. (A) Membrane vesicle (MV) proteins were isolated and visualized by Coomassie staining. Molecular size marker (in kDa) is indicated. SDS-PAGE is representative of three biological replicates (N = 3). (B) Strains were negatively stained with phosphotungstic acid. TEM images were taken in several fields at 6.000 x and 25.000 x. Images are representative of three biological replicates (N = 3).