Fig. 7

Fig. 7 Some developmental markers are dysregulated in the ventricular zones of lakritz mutants.

The zones lining the ventricles (indicated) in the midbrain and forebrain contain proliferative and differentiating cell populations. Overall patterns are unchanged in lakritz mutants (c, d, f) compared to wildtype (WT; a, b, c), although expression levels are altered. See also Table S3. The marker of terminally differentiated glia, fabp7, is downregulated in lakritz mutants (a, d). The genes encoding the mitogen Shha (b, e) and its receptor Patched1 (c, f) are reduced in lakritz mutants. Maximum projections of HCR-FISH labeling patterns from the same co-registered substacks are shown to allow direct comparisons of local expression levels across fish. Scale bar = 100 µm. All experiments were repeated three times with similar results.