ZFIN Image: Nishida et al., 2023, FIGURE 7

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Figure Caption

FIGURE 7

Alteration of CREB/BDNF pathway in GHes-fed zebrafish. Control- or GHes-fed zebrafish (7 days) were exposed to alarm substance stress. The brains were excised 15 min after the stress exposure. (A) Brain lysates were subjected to Western blotting with an anti-Creb antibody. β-actin was used as an internal control. Quantitative analyses of the intensities of the protein bands were conducted, and the results are presented as Creb/β-actin. n = 5. (B–E) Fish brains were excised 15 min after exposure to stress. (B)bdnf,(C) trkb, (D)nrf2, and (E)keap1 mRNA levels in zebrafish brains were assessed using real-time PCR. Each level of gene expression in GHes-fed zebrafish was relative to that in control. n = 8. n.s., not significant. Results are shown as means ± standard deviation.

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